1) The molecular biotechnology revolution (Restriction endonucleases - types,
nomenclature and recognition sequences - and modifying enzymes
2) Cloning and vectors (plasmids, phages, cosmids, BACs and YACs artificial
chromosomes, applications)
3) Nucleic acids isolation (principles of gel electrophoresis). Blotting methods
(Southern, Northern, Western) and applications.
4) Genomic and cDNA libraries (construction, screening and positive clones selection)
5) Genomics. DNA sequencing (Sanger method, shot-gun sequencing, NGS).
Microarrays
6) Polymerase chain reaction (PCR) (principle, types -Reverse Transcription PCR &
Real-Time PCR- and applications)
7) Transcriptomics and metabolomics
8) Gene expression systems (expression vectors)
9) Gene transfer methods to animal cells (Chemical, mechanical physical and
biological. Viruses and baculoviruses )
10) Animal engineering (methods for transgenic mouse, mammals and birds
generation). Gene transfer in Xenopus . Gene transfer to invertebrates: Transgenic
flies. Gene therapy
11) RNA interference (RNAi) and applications. Bioinsecticides
12) Genome analysis and comperative genomics (DNA, RNA and protein databases)
1) Ανασυνδυασμένο DNA (2010) Συγγραφέας: Watson D.A. κα
Εκδοτικός Οίκος: Ακαδημαϊκές εκδόσεις Ι Μπάσδρα & ΣΙΑ ΟΕ
ISBN: 978-960-88412-5-3
2) Σημειώσεις του μαθήματος ΤΕΧΝΟΛΟΓΙΑ ΑΝΑΣΥΝΔΥΑΣΜΕΝΟΥ DNA
Δ. Μηλιώνη, Π. Χατζόπουλος
