The course introduces students to recombinant DNA technology, omics technologies and their applications in the life sciences. It covers fundamental tools such as endonucleases, modification enzymes and cloning systems (plasmids, bacteriophages, cosmids). Methods for nucleic acid isolation and analysis, DNA library construction, and large-scale genomic approaches (sequencing, microarrays) are thoroughly examined. Key topics include polymerase chain reaction (PCR) and its applications, transcriptomics and metabolomics. The course also focuses on mutagenesis, gene expression systems, and DNA transfer methods in animal cells. Additionally, it addresses genetic modification of organisms, RNA interference, and comparative genomics. The objective of the course is to provide a comprehensive understanding of molecular tools, foster critical thinking and enable students to apply their knowledge to solve biological problems.