The course provides an in-depth understanding of the mechanisms underlying the regulation of genetic information flow in eukaryotic organisms, focusing on modern molecular approaches and analyses. The structures and function of the genome, analysis of gene arrays, repeats and pseudogenes, as well as intracellular transport and protein processing are examined. Students will study molecular mechanisms such as DNA recombination, mechanisms of DNA damage, repair and mutagenesis, transcription activation and RNA splicing, as well as the regulation of gene expression through epigenetic mechanisms. Particular emphasis is given on epigenetic changes in chromatin structure, the importance of epigenetic inheritance and post-translational modification of proteins. The course also covers specialized topics such as gene expression signaling, oncogenes and cancer, and the molecular mechanisms underlying cell cycle regulation and development.