Theory: Review of the structure of biological macromolecules. X-ray Diffraction.
Crystals. Crystallization. Theory of x-ray diffraction. Reciprocal space.
Crystallographic symmetry. Structure factors and Intensities. Data Collection.
Electron Density Function. Approaches to the Phase Problem. Structure refinement.
Radiation scattering from solutions of macromolecules. Thermodynamics and
Biochemistry. Calorimetric methods. Molecular Mechanics, NMR Spectroscopy,
circular dischroism, fluorescence spectroscopy, Applications in drug design and
Nanotechnology
Laboratory: Determination of thermodynamic parameters for salt dissolution,
crystallization of lysozyme, diffraction experiments with lysozyme crystals, analysis
of electron density map for lysozyme-ligand complex, fluorescence microscopy
image processing
1. Principles in Physical Biochemistry (van Holde, Johnson, Ho) 2nd Edition
2. Themata Moriakis Biofysikis (Hamodrakas) Symmetria publications
