I. Introduction and history of Model Organisms
II. From genes to genomes
III. Escherichia coli (E. coli) as a model organism
IV. The yeast Saccharomyces cerevisiae as a model organism
V. Caenorhabditis elegans as a model organism
VI. Drosophila melanogaster as a model organism
VII. Mus musculus as a model organism: advantages and applications
VIII. Zebrafish Danio rerio: advantages and applications
IX. The plant model Arabidopsis thaliana: advantages and applications
X. Beyond the classic model organisms
XI. Lab practicals: Using the Genome data viewer online tool at NCBI for analysis of genes, chromosomes, and genome comparison. Use of the OMIM (Online Mendelian Inheritance in Man) database at NCBI. Designing primers for PCR using the online tools Primer3 and primerBLAST. Design and preparation of samples for Sanger sequencing, evaluation of sequencing results. Experimental design of gene inactivation with the CRISPR / Cas9 method through appropriate online tools. Laboratory demonstration of Drosophila. Managing references with the bibliography tool Mendeley. Critical reading of a scientific publication.
Learning outcomes
Bibliography
Genetics: From genes to genomes. Fourth Edition. Hartwell Leland, Hood Leroy, Goldberg Michael,
Reynolds Ann, Silver Lee. McGrawHill Education.
Biotechnology is a rapidly advancing discipline which aims at exploitting the progress in life and physical sciences as well as other related fields, in developing new and advanced products, processes and services
