AGRICULTURAL
UNIVERSITY OF ATHENS
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Cell Culture Technology

Cell Culture Technology

Content

1. Processes of cell division and differentiation or dedifferentiation in vitro.
2. Nutritional culture media and growth factors.
3. Creating aseptic conditions and handling under them.
4. Micropropagation. Problems associated with the process of plant tissue culture.
5. Induction of random organogenesis or somatic embryogenesis, development and maturation of somatic embryos. Creation of synthetic seeds.
6. Protoplast isolation, culture and fusion. Anther culture and production of diploid plants. Cultivation of ruminants, buds and embryos.
7. In vitro tissue preservation, in vitro fertilization, in vitro selection, micro inoculation, in vitro flowering.
8. Bioreactors.
9. Bioactive Compounds production from cell/tissue cultures. Immobilization of cells and culture of immobilized cell systems. Production and cultivation of alder roots.
10. Design and operation of a micro propagation laboratory.
11. Types of mammalian cells.
12. Mammalian Cell Tissue Culture Applications.
13. Organization of a basic unit of animal cell cultures.
14. International Cell and Tissue Culture Collections.
15. 3D culture.
16. Mammalian cell culture systems. Primary cultures. Cell lines. Dοubling time. Infections.

Learning outcomes

Bibliography

• Σπύρος Κίντζιος, Εισαγωγή στον Μικροπολλαπλασιασμό των Φυτών, Ελληνικά Ακαδημαϊκά Ηλεκτρονικά Συγγράμματα και Βοηθήματα (http://repository.kallipos.gr/handle/11419/241), 2015, ISBN 978-960-603-033-8
• Landecker Hannah, Η καλλιέργεια της ζωής στο εργαστήριο. Πώς τα κύτταρα έγιναν τεχνολογία, Εκδόσεις Πατάκη, 2017, ISBN: 9786188323841

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Biotechnology is a rapidly advancing discipline which aims at exploitting the progress in life and physical sciences as well as other related fields, in developing new and advanced products, processes and services
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