1. Safety management of analytical laboratory.
Safety rules for analytical biotechnology laboratory.
Disposal and waste management.
Rules of good laboratory practice.
2. Evaluation and Treatment of Analytical Data.
Introduction to sampling and chemometrics for the optimization of analytical measurements.
Reliability of analytical methods.
Quality assurance and quality control of analytical data.
Errors and statistical treatment of analytical data.
Selection criteria and conditions for the development of analytical method and laboratory protocol.
3. Sample preparation.
Fundamentals of extraction - separation of biomolecules (wet extraction methods, solid phase
extraction, extraction with supercritical fluids, ultrasound - microwave and, enzyme assistant
extraction, dialysis, ultrafiltration, lyophilization).
4. Methods and techniques.
Chromatography (adsorption, distribution, gas chromatography, liquid chromatography)
Combined techniques (LC / MS, GC / MS , etc. )
Electrophoretic analysis methods (isoelectric focusing, two-dimensional electrophoresis, capillary
electrophoresis, immunoblotting)
Centrifugation techniques
Enzymatic methods of analysis
Analytical nanosystems and protein nanostructures
Proteomics analysis
Peptide synthesis and carbohydrate/lipids analysis
Characterization of protein complexes
5. Applications.
Specific applications and examples of analytical methods and protocols for biomolecules with
agricultural, industrial and clinical interest.
Learning outcomes
Bibliography
• David G. Watson (2011), Φαρμακευτική Ανάλυση, Επιστημονικές Εκδόσεις Παρισιάνου Α.Ε.
• P.R. Yadav (2005) Experimental Biotechnology, Discovery Publishing House.
• Rodney F. Boyer (2000), Modern Experimental Biochemistry (3rd Edition) Addison Wesley
Longman.
• Keith Wilson and John Walker (2000) Principles and Techniques of Practical Biochemistry (5th
edition), Cambridge University Press.
• Clark jr. J.M. and Switzer R.L., (1992) Πειραματική Βιοχημεία, Πανεπιστημιακές Εκδόσεις Κρήτης,
1992.
• Holme D. and Peck H. (2001) Analytical Biochemistry, Pearson Education Editions.
Biotechnology is a rapidly advancing discipline which aims at exploitting the progress in life and physical sciences as well as other related fields, in developing new and advanced products, processes and services
