1) Applied enzyme kinetics.
2) Downstream processing / enzyme purification technology (classification of enzymes and enzyme
sources, the purification protocol, solid-liquid separation, cell disintegration, low purification stage /
fractionation, high purification stage / chromatographic techniques, enzyme formulation and quality
control, examples on enzyme purification).
3) Immobilized biocatalysts (methods and techniques, influence of immobilization on the enzyme’s
molecular and kinetic characteristics).
4) Enzyme applications in the food industry (starch, bakery, beer, wine, fruit juices, vegetable oils,
cheese, lactose).
5) Large scale enzyme applications (paper, textiles, leather, home laundry detergents, animal food).
6) Enzyme applications in the chemical industry (aminoacids, pesticides, oligosaccharides, chemicals,
food supplements).
7) Enzyme applications in the pharmaceutical industry (antibiotics, steroids, drugs against hyper
cholesterolhaimia, HIV, hypertension, etc).
8) Enzyme applications in the analysis (the enzymes as reagents and as markers, enzyme-lined
immunosorbent assays, enzyme biosensors).
9) Enzyme catalysis in organic solvents (applications in water-miscible and water-immiscible solvents,
aromatic products, pesticides, triglycerides, peptides, insulin, aspartame, etc).
10). Discovery of new enzymes.
1). Y.D. Clonis, Enzyme Biotechnology, Crete University Press, Heraklion, Crete, Greece, 3rd revised
edition, 2013.
2). K. Buchholz, V. Kasche, U.T. Bornscheuer, Biocatalysis and Enzyme Technology, Wiley-VCH Verlag
GmbH, Germany, 2005.
3). G. Walsh, Proteins: Biochemistry and Biotechnology, John Wiley & Sons Ltd., Chichester, UK, 2002.
