European & Developing Countries Clinical Trials Partnership (EDCTP) - Horizon 2020 "Research and Innovation Actions" DESCA

The overall goal of this project is to develop biochemical treatments that adjust native EBOV GP in patient sample as a target for rapid diagnostic testing.  Four specific aims are contingent to this goal, viz: (i) pre-treatment with various mixtures ( namely a. glycosidases to remove glycans, b. endopeptidases to denature secondary structure, and c. reducing agents to break disulfide bond)  to expose target conserved epitopes, (iii) Optimization of concentrations of buffer mixture (iv) ROC-characterization of the optimized sandwich EIA for filovirus rapid diagnostic testing, (iv) Prototype testing within the setting of the on-going EVD outbreak in Kivu, Democratic Republic of Congo-DRC.