AGRICULTURAL
UNIVERSITY OF ATHENS
Department of Food 
Science & Human Nutrition

Instrumental Chemical Analysis

Content

1. Introduction to Instrumental Chemical Analysis
2. Sampling- Sample preparation before analysis
3. Extraction techniques
4. Distillation techniques
5. Chromatographic analysis techniques
6. Gas Chromatography (GC)
7. High Performance Liquid Chromatography (HPLC)
8. Spectroscopic analysis techniques
9. Ultraviolet-Visible Spectrophotometry (UV-Vis)
10. Infrared Spectroscopy (IR)
11. Raman spectroscopy
12. Nuclear Magnetic Resonance Spectroscopy (NMR) ( 1H, 13 C NMR)
13. Mass Spectrometry (MS)
14. Exercises

Laboratory exercises:
Exercise 1 Electronic-computer and network technologies in Chemistry - Stereochemistry.
In silico study.
Exercise 2 Ultraviolet Visible Spectroscopy
Quantification of quality characteristics of saffron.
Exercise 3 Infrared Spectroscopy
Determination of functional groups-identification of organic compounds using infrared spectroscopy.
Exercise 4 Raman Spectroscopy
Determination of ethanol and methanol in alcoholic beverages.
Exercise 5 Nuclear Magnetic Resonance Spectroscopy
Determination of compound structure-Interpretation of spectra.
Exercise 6 Obtaining essential oil by distillation
Exercise 7 Gas chromatography – Mass spectrometry
Qualitative and quantitative determination of essential oil components by gas chromatography.
Exercise 8 Liquid chromatography
Separation and quantification of caffeine in soft drink stimulants by high performance liquid chromatography.

Learning results

The objective of the Instrumental Analysis course is to acquaint the students with the modern methods of instrumental chemical analysis at a theoretical and practical level. More specifically in the theoretical courses, emphasis is placed on the principle of each method, on its organization, on the interpretation of the provided graphs or spectra, as well as on the processing of the results for qualitative and quantitative measurements. Particular emphasis is placed at the end on the choice of method/or methods for solving specific analytical practical problems from research or from industrial practice.

The objective of laboratory exercises is to familiarize students with the organization and applications of Instrumental Analysis Methods (which are usually used in research laboratories, in public or private control laboratories). At the same time, laboratory exercises are aimed to:

  • The direct connection of theoretical knowledge with practical application.
  • Learning how to properly prepare the sample before analyzing it.
  • The understanding of the basic operational parameters on which each method of instrumental analysis depends.
  • Learning the correct process of measuring, receiving, processing the data and estimating the final result.

At the same time as the previous ones, students are trained in writing laboratory reports.

Bibliography

1. Principles of Instrumental Analysis. D. A. Skoog, F. James Holler, T. A. Nieman (Translation: M. I. Karagiannis, K. I. Efstathiou, N. Haniotakis).
2. ANALYTICAL CHEMISTRY, G. Christian, P. Dasgupta, K. Schug, Odysseus Publishing Ltd.
3. INSTRUMENTAL ANALYSIS, G. N. Jill, G. II M. Robert, Y. M. Hank, S. D. Karl, Broken Hill Publishers.
4. ANALYTICAL CHEMISTRY, LIODAKIS STYLIANOS, A. PAPASOTIRIOU & SIA I.K.E., 2nd edition.
5. P. A. Tarantilis, M. Polysiou, C. Pappas. Instrumental Chemical Analysis, University Notes.

Faculty

(+30) 210 529 4262
ptara@aua.gr
Instrumental Chemical Analysis of Natural Products
(+30)-210-5294262
chrispap@aua.gr

NEWSLETTER

The Department of Food Science and Human Nutrition (renamed Department of Food Science and Technology, Decree 80/27/5/13, Government Gazette A119 28/5/13) offers its students the scientific background for a rational approach to scientific and technological issues related to the food sector.
linkedin facebook pinterest youtube rss twitter instagram facebook-blank rss-blank linkedin-blank pinterest youtube twitter instagram Skip to content